Our Laboratory

Tests And Technologies

Testing

WHO endorsed TB tests; phenotypic tests consist of Microscopy  (ZN and Fluorescence), liquid culture (MGIT 960, BD), and genotypic testing consist of CBNAAT (GeneXpert MTB/RIF) and Line probe assay (LPA). In the ‘Focus TB’ platform, these tests are made available at affordable prices to patients.

Technologies We Use

Direct ZN microscopy of sputum smears are done to quickly detect the TB infection. Mycobacteria has a distinguished thick lipid cell wall that remains stained even after washing with acid-fast reagents. This a simple and an inexpensive method for quick detection of acid-fast bacilli.

Fluorescent microscopy is more sensitive than ZN microscopy for the detection of TB bacteium.

Liquid and solid cultures are used as definitive diagnosis of MTBC and to distinguish it from other Mycobacterial species. DST technique is used to test for resistance of TB bacteria to certain drugs.

At Thyrocare, we are performing liquid culture by MGIT 960 and drug  susceptibility testing for 13 drugs with critical concentrations recommended by WHO.

This is a rapid molecular diagnostic technique with high sensitivity for TB diagnosis in sputum and other body fluids. This test uses DNA amplification for detection of M. tuberculosis.

This is one of the latest techniques in the field of TB diagnostics. The need for fast and accurate results is ever increasing for TB diagnosis and it is also important to determine drug resistance; GeneXpert MTB/RIF comes as an ideal solution for high sensitivity and specificity. This technology helps in detecting simultaneously M. tuberculosis complex (MTBC) and rifampin resistance .

The GeneXpert MTB/RIF test is a cartridge based nucleic acid amplification assay (CB-NAAT) which uses real time-PCR for detection of an MTB-specific region of rpoB gene. It detects mutations associated with rifampicin resistance.

Multi-drug resistance TB poses a potential threat to TB control due to its treatment challenges. Line probe assay is a novel technology for rapid detection of anti-TB drug resistance, in first line and second line drugs. This assay involves extraction of M. tuberculosis DNA from clinical specimens and subjecting it to PCR for the amplification of the resistance determining regions of the gene using biotinylated primers. After amplification, the labelled PCR products are hybridized to specialized probes that are immobilized on a strip. The hybrids fixed on the strips are detected by colorimetric development, detecting the presence of MTBC and mutant genes for resistance.

FOCUS TB Test

List of Tests and Rates

No. Description Rate(INR)
1 TB PCR 1200
2 GENEXPERT 2200
3 TB MDR SCREEN (LPA) 1800
4 TB XDR SCREEN (LPA) 1800
5 SMEAR AFB 300
6 FLUORESCENT SMEAR 350
7 AFB CULTURE (MGIT) 900
8 AFB DRUG SUSCEPTIBILITY (SIRE) 1ST LINE 1700
9 AFB DRUG SUSCEPTIBILITY (SIREP) 1ST LINE 3000
10 AFB DRUG SUSCEPTIBILITY (KEPO) 2ND LINE 3000
11 AFB DRUG SUSCEPTIBILITY (MACC) 2ND LINE 3000
12 AFB DRUG SUSCEPTIBILITY (13 DRUGS) 7000
13 AFB DRUG SUSCEPTIBILITY – MOTT 4000
14 ANTIBIOGRAM – MTB (AMIKACIN) 2000
15 ANTIBIOGRAM – MTB (CAPREOMYCIN) 2000
16 ANTIBIOGRAM – MTB (CLOFAZIMINE) 2000
17 ANTIBIOGRAM – MTB (ETHAMBUTOL) 2000
18 ANTIBIOGRAM – MTB (ETHIONAMIDE) 2000
19 ANTIBIOGRAM – MTB (KANAMYCIN) 2000
20 ANTIBIOGRAM – MTB (LINEZOLID) 2000
21 ANTIBIOGRAM – MTB (MOXIFLOXACIN) 2000
22 ANTIBIOGRAM – MTB (MOXIFLOXACIN HIGHER DOSE) 2000
23 ANTIBIOGRAM – MTB (OFLOXACIN) 2000
24 ANTIBIOGRAM – MTB (PAS) 2000
25 ANTIBIOGRAM – MTB (PYRAZINAMIDE) 2000
26 ANTIBIOGRAM – MTB (RIFABUTIN) 2000
27 ANTIBIOGRAM – MTB (RIFAMPICIN) 2000
28 ANTIBIOGRAM – MTB (STREPTOMYCIN) 2000
29 ANTIBIOGRAM – MTB (ISONIAZID) 2000
30 TB SCREENING PROFILE 2000

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